Replicating Genetic Cloning To Produce Red Fluorescent...

Replicating Genetic Cloning to Produce Red Fluorescent Protein Introduction: Genetic engineering has been used by scientists to create proteins in different amounts to adapt bacteria and now students will have the chance to learn and replicate different procedures (Amgen Biotech Experience, 2015). One recent discovery was that because of genetic engineering you can release insulin at your own free will through your phone. This was tested on a diabetic mouse that had an implant that was shown with an LED light (Shao et al./Sci. Trans. Med. 2017). One of the most important tools used in this lab is a micropipette. A micropipette is a device used to transport different amounts of liquids into another compartment by means of dispensing it.†¦show more content†¦Add restriction buffer and the plasmids into two tubes labeled R+ and R-, restriction enzymes into R+, and distilled water into R-. One thing to note is to mix after you add anything to tubes and once it is finished let the two tubes spin in a microcentrifuge for about four seconds in order to fully mix it all. After this, place the two tubes in a rack and incubate them in a water bath that is 37 degrees Celsius for an hour and place them in a freezer that is -20 degrees Celsius. Lab 4A consists of using gel electrophoresis to further examine the pARA-R plasmid by viewing them in a DNA ladder. Start by adding loading dye into the two tubes and centrifuging them for a few seconds. Before putting them in the gel walls check to make sure it is in the negative side an d not the positive side. Add buffer to cover the walls and keep track of where you will add the R-, R+, and ladder. It is very important to use new, fresh tips and to add them very carefully. Once everything has been put in, cover the box and connect the cables to the power supply in the correct order: black to black for negative and red to red for positive. Turn the power on and put the voltage up to 130v-135v and let it set for 40 to 50 minutes. For the first two to three minutes check to see if it is moving towards the positive side and if it isn’t, inform the instructor. This lab ends with the instructor providing a picture of how far the plasmids went. Next for lab 5A is putting the plasmid into E.Show MoreRelatedBCH190 Essay14810 Words   |  60 Pagesand including Chapter 2 1. Difference occurs in the sequence of long chain molecules and becomes information in biological organisms. ‘Life’ assembles itself into chains: (A) of RNA (B) all of the answers are correct (C) of DNA (D) of protein (E) none of these answers are correct 2. Which of the following foods is not a significant source of complex carbohydrates? (A) fresh fruit (B) rice (C) pasta (D) oatmeal (E) all of the above are significant sources of complex carbohydrates Read MoreCell Biology Final Essay30093 Words   |  121 Pagesinitial genetic system because it can A) form a stable double helix with a complementary nucleic acid strand. -B) catalyze the polymerization of nucleotides into another RNA strand. C) form ribosomes. D) transfer amino acids to ribosomes. 9. The initial importance of a membrane enclosing self-replicating RNA molecules and associated proteins was that a membrane -A) maintained these molecules as a unit capable of reproduction and evolution. B) provided sites for proteins to function

Themes Of Unity In The Grapes Essay - 1489 Words

John Steinbecks novel, The Grapes of Wrath, is a moving account of the social plight of Dustbowl farmers and is widely considered an American classic. The novel takes place during the depression of the 1930s in Oklahoma and all points west to California. Steinbeck uses the Joad family as a specific example of the general plight of the poor farmers. The Joads are forced off of their farm in Oklahoma by the banks and drought, and they, like many other families of the time, head out for the promised land of California. They endure much hardship along the way, and they finally make it to California only to find that work is scarce and human labor and life are cheap. Tom Joad, the eldest son in the family, starts the book freshly out of jail†¦show more content†¦Tom retaliates in rage, killing a deputy, and forcing him into hiding. Alone all day long for weeks, he begins to think about the plight of the migrant workers and about what Jim was constantly babbling about. One thing t hat Jim Casy said close to his death, which Tom broads upon, has to do with a revelation that came to the preacher while he was in jail. He tells a group of followers amp;#8230;one day they give us some beans that was sour. One fella started yellin, an nothin happenedamp;#8230; then we all got to yellinamp;#8230; By God! Then something happened! They come a-runnin, and they give us other stuff to eat; (522). This was a telling example to Tom about the power of the group over one man. Tom reveals his thought evolution in the final meeting with his mother, before he leaves to continue the work of Jim Casy. He says, But I know now a fella aint no good alone,; and Two are better than one, becauseamp;#8230; if they fall, the one will lif his fellow, but woe to him that is alone when he falleth, for he hath not another to help him up; (570). Peter Lisca, an extensive critic of Steinbecks work, explains amp;#8230;in is last meeting with his mother, in which he asserts his spiritual unity w ith all men, it is evident that he has moved from material and personal resentment to ethical indignation, from particulars to principles; (Lisca 98). Tom clearly changesShow MoreRelatedThemes of Unity in the Grapes1599 Words   |  7 PagesJohn Steinbeck#8217;s novel, The Grapes of Wrath, is a moving account of the social plight of Dustbowl farmers and is widely considered an American classic. The novel takes place during the depression of the 1930s in Oklahoma and all points west to California. Steinbeck uses the Joad family as a specific example of the general plight of the poor farmers. The Joads are forced off of their farm in Oklahoma by the banks and drought, and they, like many other families of the time, head out for theRead More Grapes of Wrath Essay: Theme of Strength Through Unity987 Words   |  4 Pages Theme of Strength Through Unity in The Grapes of Wrathnbsp;nbsp; The traditional human family represents a necessary transition between self and community. In the difficult era of the 1930s, the familys role shifted to guard against a hostile outside world rather than to provide a link with it. With the drought in the Dust Bowl and other tragedies of the Great Depression, many were forced to look beyond the traditional family unit and embrace their kinship with others of similar necessityRead MoreScott Fitzgeralds The Great Gatsby and John Steinbecks The Grapes of Wrath1720 Words   |  7 PagesGreat Gatsby and John Steinbecks The Grapes of Wrath In the novels The Great Gatsby by Scott Fitzgerald and The Grapes of Wrath by John Steinbeck, the authors present similar ideas, but use different methods to portray them. Similarities in themes can be made between the two texts; these include the pursuit of the American Dream and the use and misuse of wealth. Other themes are also central to each novel, the strength in unity and the influence of female charactersRead More Grapes of Wrath Essay: Steinbecks Communist Manifesto1071 Words   |  5 PagesThe Grapes of Wrath as a Communist Manifesto  Ã‚  Ã‚        Ã‚  Ã‚   Steinbecks political views are quite evident within The Grapes of Wrath. The subject of much controversy, The Grapes of Wrath serves as a social protest and commentary. Steinbecks views as expressed through the novel tie directly into the Marxist ideals on communism.    Perhaps the first thing Steinbeck does in The Grapes of Wrath is establish the status quo. He sets up the farmers and the banks as the two main opposing forcesRead MoreBiblical Allusions to The Grapes of Wrath Essay example1457 Words   |  6 PagesBiblical Allusions to The Grapes of Wrath John Steinbeck was born in Salinas, California, on February 27, 1902. He studied marine biology at Stanford University and then traveled east on a freighter through the Panama Canal. Steinbeck went to New York to work as a newspaper reporter but soon returned to California and held a variety of jobs while he wrote. Steinbeck published Tortilla Flat in 1935, Of Mice and Men in 1937, and The Red Pony in 1937, which established his reputation as a forcefulRead MoreRole Of Robert Steinbeck s The Grapes Of Wrath 1286 Words   |  6 PagesRobby LaRoy APLang 2 º Ms. Lehman 9/13/14 The Role of Unity in Survival During the great depression in the plains of Oklahoma, workers were forced out of their homes as their crops withered away to nothing and dust took over. The general feeling of these migrant workers during the late 20’s and early 30’s can be summarized by the struggle for survival showcased in The Grapes of Wrath by John Steinbeck. In the novel, a family called the Joads makes their way westward in hopes of a better life forRead MoreThe Grapes Of Wrath By John Steinbeck1967 Words   |  8 PagesThe Grapes of Wrath by John Steinbeck is the story which takes place in a time where opportunity is nil and desperation has overtaken American en masse. The story is told several viewpoints with majority of them being witnessed from Tom Joad and his family while others are from minor characters who share a similar circumstance or actively benefiting from the misfortunes of others. Larger businesses are overtaking others and placing profits above people. The care and welfare for other human becomesRead MoreSymbolism Of The Steinbeck s The Grapes Of Wrath 2259 Words   |  10 Pages Symbols In The Grapes of Wrath, the Joad family experiences many hardships on the journey to and in California, ranging from dying family members to a lack of sufficient food. In the third chapter of the novel, author John Steinbeck introduces a determined turtle who attempts to make its journey across a highway. The turtle is apparently nearly run over multiple times, and is actually hit by a car. This causes the turtle to be flipped on its shell, until it catches its footing and â€Å"littleRead More The Grapes of Wrath as Communist Propaganda Essay example1199 Words   |  5 Pages The Grapes of Wrath as Communist Propaganda nbsp; The Grapes of Wrath may be read as a direct indictment of the U.S. capitalist system of the early and mid twentieth century. Although the book on the surface level can fairly easily be read as anti-capitalist book, it goes further than that. The book both implicitly and explicitly advocates structural changes in the economic institutions of our country. Thus, it may be argued that the Grapes of Wrath is communist propaganda. Propaganda, accordingRead More Grapes Of Wrath Biblical Allusions Essay889 Words   |  4 Pages John Steinbeck carefully molded his story The Grapes of Wrath to encompass many themes and ideas. He included several Biblical allusions to enforce his message of the migrating families coming together to form a community. Steinbeck alludes to Biblical characters through Jim Casy and Rose of Sharon, events like the family’s journey to California and the flood at the end of the novel, and teachings throughout the novel.   Ã‚  Ã‚  Ã‚  Ã‚  The Biblical allusions represented by the characters in the novel

Analysis of Pericles Speeches Free Essays

Although Thucydides provides a history of the Peloponnesian War, Thucydides spends a lot of time emphasizing the government and the citizens of Athens. Thucydides also focuses on the greatness of Pericles and his honest and patriotic leadership. Does this concentration on a democracy and moderate leader reveal what Thucydides sees as a just government? Throughout the text, one can see clues that a democracy is the preferred government of Athens, and the focus on Athens as the greater, swifter and more innovative power; he contradicts them with the passive, slow and cautious Sparta. We will write a custom essay sample on Analysis of Pericles Speeches or any similar topic only for you Order Now This text not only acts as a historical resource but also a source that provides an example for future governments on how to run a government successfully. By looking at the speeches of Pericles, the view of Thucydides comes through. It seems that Thucydides believes in a democracy and giving the common man a voice in what happens in the city. Pericles emphasizes the greatness of a democracy in his funeral oration. Through his speech Pericles emphasizes that equality to create a free and law-abiding society. I think Pericles speaks of how there is no class consideration when considering man to be able to serve the state because he wants to stress that the most honorable man is the one that serves the state, especially in a time of war. Citizens that do not participate in the duties of public matters are deemed ‘useless. ’ The funeral oration is not just an honoring of the patriotic men that died fighting for Athens but also a call to all the other sons, brothers and fellow citizens of the honorable fallen to live a life as honorable as them. A democracy is a form of government that gives all the ability to participate, and according to Pericles everyone has a responsibility to take part. Men must put aside their petty wants and look at what is best for the state as a whole. The state as a whole can bring a lot to the people but a single person acting on their own individual interests can do little for the state. Why are there so many of Pericles’ speeches in this text? Was Thucydides giving the prime example of what a leader should be? Was the emphasis on Pericles putting a characteristic of him above those of the other men and leaders throughout the war? It is clear that Thucydides found all leaders succeeding Pericles as poor leaders. In Book 2. 65, Thucydides talks of how the leaders following Pericles were striving for supremacy, and acting according to their own private ambitions and interests. By going contrary to the moderate policy Pericles emphasized, a plan including exposing themselves to no hazards and waiting quietly while keeping attentive to their marine, the war was ultimately lost by the Athenians. Were the speeches recorded to also show how powerful a leader can be? When Athens held an assembly to determine whether they would concede to the concessions of Sparta, it was the speech of Pericles that invigorated the Athenians with confidence to declare war against Sparta. When the plague hit the Athenians and they had lost momentum in the war effort and were considering sending peace envoys to Sparta, it was a speech by Pericles that was able to increase the Athenian energy to the war. A great leader has the ability to drive the weak masses to great heights when they know they are under a first-rate leader and is told of the superiority of their state; this is seen in the example of Pericles. As a reader, I feel that Athens can win the war with their advantageous sea power, lands across the sea that Sparta cannot harm. By including the speeches of Pericles in his text, Thucydides sets an example of what is a just form of government and what makes a good leader in a democracy. How to cite Analysis of Pericles Speeches, Essay examples

Phenology as a Measure of Climate Change

Question: Discuss about thePhenology as a Measure of Climate Change. Answer: Introduction Phenology is the study of the cycle of lives of animals and plants in relation to the environmental conditions. Climatic change has brought about shifts in phenology in many species of plants and animals in Australia just like across the whole world (Hannah, 2011). There has been no ideal yardstick for measuring the shift, but in some cases, the change in the abundance of an organisms food and other natural supplies is used to measure the change. Issues such as migrations of animals and birds from one region to another show how the climatic changes have impacted phenology in Australia. Due to the climatic change in Australia, there has been a significant shift in the time when the four main seasons occur. The temperatures have been observed to rise earlier than before in spring and stay warmer even after the kick-off of the fall season (Lerner Lerner, 2008). By monitoring the plants and noting when they bud and flower as well as when their leaves fall one can clearly see that there is a significant effect of the climatic change on the plant life and this can be used to figure out seasonal patterns and also help scientists to predict the future. The migration of different species of fishes is another aspect that can be utilized as a measure of climate change in Australia. There is an observable difference between the timings that the turtles and birds migrated from one region to another. Currently, the migrations happen earlier or later than they used to occur in the earlier days and this is because of changes in the climatic conditions (Hudson Keatley, 2010). The destinations, in which the migrations used to have, also have significantly changed as a result of the shift in the environmental conditions in water and land. The observable extinction of some species of plants and animals is a measure of climatic change in the world. Some animals that existed some years ago have all died because the climatic conditions were no longer fit for their survival (Souter Lewthwaite, 2007). One familiar example is the extinction of the desert rat-kangaroo in Australia decades ago. Studies show that some plant species have also got to the level of extinction as a result of the shift in the climatic conditions (Potential effects of climate change on forests and forestry in Australia, 2011). Close observance of the extinction patterns in both plant and animal life can be used by scientists to gauge the climatic changes. The introduction of new species of plants and animals is a measure that can be used to trace the climatic change across Australia. New species of plants have started growing and thriving in areas that they never existed before (Morison Morecroft, 2006). Close observation of the new species in both plant and animal life in Australia can be used to trace the changes in the climate and also help to foretell the future occurrences. References Hannah, L. (2011). Climate change biology. Burlington, MA: Academic Press. Hudson, I. Keatley, M. (2010). Phenological research. Dordrecht: Springer. Lerner, B. Lerner, K. (2008). Climate change. Farmington Hills, Mich.: Gale, Cengage Learning. Morison, J. Morecroft, M. (2006). Plant growth and climate change. Oxford: Blackwell Pub. Noormets, A. (2009). Phenology of Ecosystem Processes. Dordrecht: Springer. Potential effects of climate change on forests and forestry in Australia. (2011). Canberra, A.C.T. Rumpff, L. (2008). Potential biological indicators of climate change. Melbourne: Victorian Dept. of Sustainability and Environment. Schmidt, G., Scho?nrock, S., Schro?der, W. Plant phenology as a biomonitor for climate change in Germany. Schwartz, M. Phenology. Souter, N. Lewthwaite, K. (2007). Nature in a changing climate. Hatfield, Herts.: Association for Science Education.

The Greenwashing Effect - The Writers For Hire

THE GREENWASHING EFFECT Copyblogger’s â€Å"Four Ways to Target Online Buyers with the Right Words† has a great analysis/advice section for marketing to an eco-friendly, socially conscious audience  (among other types of consumers). Green is the new black, and it seems a lot of businesses are trying to revamp their marketing efforts to promote a more â€Å"green† brand. But some of these companies are just using increased interest in protecting the environment as a cheap advertising and PR marketing opportunity. Greenwashing – deliberately misleading customers about the environmental practices or ingredients in a product – has become so prevalent in recent years that the Federal Trade Commission has Green Guides in place to help marketers steer clear of false and misleading claims. So as the American consumer becomes more jaded in this greenwashed society – especially when corporations like Shell, BP, and GE are regularly accused of greenwashing their marketing campaigns – here are some tips about giving treehuggers what they want to hear (and generally just good marketing advice): 1. Be Specific Forget generic claims like â€Å"All-natural† – it’s an obvious greenwashing term that means nothing. But, if you can be very, very detailed about your product, your company may gain some points in the green revolution. Consider the difference between labeling your product as â€Å"All-natural† versus â€Å"Our products are grown at our farms in Greensburg, Kansas. We don’t use pesticides or toxic chemicals of any kind, and we harvest everything ourselves so that we can deliver our best product straight to you.† The latter is obviously more detailed, and seemingly more reliable than the nebulous, broad-reaching, unsubstantiated claim. 2. Be Relevant Don’t include irrelevant â€Å"eco-friendly† features about your products – unless your product is new or the feature is new. For example, claiming that your paper towels are â€Å"100% Biodegradable† is irrelevant – all paper towels are biodegradable. And a certain major beverage company, in an advertisement for their new â€Å"eco† water bottle, claims that the bottle is â€Å"100% Recyclable† – which is great, but the bottle has always been recyclable, and all plastic bottles are recyclable anyway. Don’t drive people away with silly or misleading information; instead, include information like this only if your product is new or improved. 3. Be Transparent In other words, don’t try to hide anything. If you want to gain reputability in the green community, always refer consumers to your website on your product label – true eco-conscious consumers will check! Make sure everything, such as all ingredients used, your refinement and manufacturing processes, company affiliates and associations, philosophy, and pictures, are clearly spelled out on your website. It’s not always easy to make a name for yourself in the green community, but after establishing your credibility and earning consumer’s respect, you’ll have a long and prosperous relationship with your devoted followers.

Oh My God Essays - Asil Chicken, Oh My God, Oh My, School Nurse

Oh My God Essays - Asil Chicken, Oh My God, Oh My, School Nurse Oh My God Story It was just like the movies, last year on Saturday morning before the Islamic exam. I was startled when I saw my best friend fainting in front of me. ?Oh my god, HELP!? ?Asil fainted!? My friends shouted, and immediately called 911. I froze, and felt that everything was dimly. The news was relayed like lightning to the entire school; therefore, everybody rushed to help her. A circle of people was immediately formed around her; every single one standing there felt condolence. Asil didn?t move for five minutes. I cried so much when the school nurse was covering her because that?s when we knew she was gone. Unexpectedly she opened her eyes gradually. It was a miracle her heart stopped for over five minutes, and started working suddenly. Everyone had a worried smiled on. The principle tried to enable her to stand, and start talking but he failed. She was oblivious of the surroundings, and extremely limped. The ambulance arrived just in time. The principle proved, in uniquely human way, that he cares about the students in the school because he carried her to the ambulance, and continually visited her at the hospital for the past year. When I first saw Asil after the school incident at the hospital, she was lying in bed tremendously sedated, but as the days, months, and year passed by she recovered. Her Anemia is all most gone, and is currently making up for all the work she missed in addition she will graduate in the year 2012.

McDonald going to Russia Coursework Example | Topics and Well Written Essays - 1250 words

McDonald going to Russia - Coursework Example The company does this by itself and in consideration of the communities around which they operate. One major factor that has made the company going is that, they have been a focused on doing the right thing just from the beginning. This has made their values define who they are, what they do and how they operate (McDonald, 2010). If McDonald would like to take its restaurant services to Russia, there are various considerations that the company management should take into consideration before doing the launch. The considerations are on the marketing issue of the company. After carrying out an adequate environmental analysis of the companys external environment, it would be wise for the company to include an analysis of how the possibility of launching its products and services in Russia. To be able to do this, the company can consider the analysis around communication, consumption, country culture, and consumer behavior. These are issues in marketing that re usually categorized under 12Cs of marketing. However; for this kind of assignment, just the five Cs are found more appropriate and will highly count when trying to launch McDonalds products in Russia. This report will majorly dwell on the discussion of the consideration of McDonalds entry into Russia and the extent to which part of the 12 Cs mention above will influence its entry into this market. The first C that McDonald would mind thinking about before deciding to enter into Russia will be on Communication. Communication in this sense will mean a lot as communication usually means a lot in any form of business. McDonald, to begin with, will have the task of knowing the major language that is being spoken u the Russians. This would be important as it is a language that facilitates communication. The essence of this is that, it is usually necessary that the company staff communicates with the customers on various issues of concern so that most of the business can be solved. The

The Metaphysics of Morals by Emmanual Kant Essay

The Metaphysics of Morals by Emmanual Kant - Essay Example Kant has spent most of his life in a German town, Konigsberg, which was located near East Prussia. He had also taught in the Konigsberg University and had maintained a descent reputation in all these years, thus by the age of fifty, much of his work on natural sciences as well as philosophy had been published and gained much momentum in the German literary community. Later in his life, Kant had attained such high regard in this context that his works stood out in comparison to Plato`s and Socrates` philosophies which was definitely an achievement for him. Moreover, Kant had the privilege to contribute for the literary community of Europe during the age characterized as the Enlightenment Era, which was an age of modernization and prosperity especially for the fields of academia, philosophy, literature etc. Thus, reasoning and logic were the dominant concepts being discussed and focused upon during the era, which is also reflected in the works of Kant. Kant`s inspiration or rather his mentor who drove him towards logic and rationality is believed to be the Scottish philosopher David Hume, who Kant himself has quoted at different points. Hume is the pioneer for incorporating causation behind everything, which implies that he believed that there is a reason behind everything.... For instance, philosophers before Kant were of the view that the â€Å"cause† behind the universe is God as He was the one who created everything. However, Kant countered this proposition by the logic that this implies just a series of events and not the cause or rather logic behind it, since logic is formed by virtue of rational connections sought by our minds. Moreover, the critical analysis posed over Kant by other philosophers is also important to understand the context of the study. Many different philosophers argue that Kant went too forward and ignorant when describing morals, and giving moral theories when using logic to develop a cause and effect relationship. These philosophers are of the view that morals should not always be defined and described with logic and rationality as it is not always fair for ethics to be believed to be rooted in rational logics. Different philosophers use logic to rather distort ethics and the scope of the field. For example, the enlighten ment era marks many such instances when the philosophers have justified imperialism in the light of moral explanations. In other words, they are of the view that it is fair enough to rule and empower the colonies as they are less intellectual and civilized than the imperialists, thus it is morally fair to civilize them morally as well as intellectually. This is a far fetching debate which will be addressed at various instances during the course of the paper. Starting off with the Metaphysics of Morals, it is first important to define the main concepts involved. Firstly, it is important to understand that there are three tenets of philosophy i.e. physics which is the physical study of beings and existence, the ethics which deals with morals and ethical norms,

Final Report (other different copy) Essay Example | Topics and Well Written Essays - 2000 words

Final Report (other different copy) - Essay Example Today Qatar Islamic Bank stands as one of the largest and finest financial institutions in the Islamic banking sector (Qatar Islamic Bank). QIB provides diverse financial services. It contains programs of the real estate investment, long-term business and industrial investment. Furthermore, there are services of retail banking and personal banking which QIB organizes. Altogether there are six segments in which QIB works inclusively project finance, contractual finance, asset, real estate, corporate and working capital finance (Qatar Islamic Bank). We believe in Islamic Shari’a and therefore serve the mission to provide Shari’a-compliant financial solutions to our clients. We dedicate ourselves to maximize efficiency, not just for our clients but also for the whole society that give us the clean opportunity to grow and to serve. It is innovation, which sets our path for performance and integrity (Qatar Islamic Bank). For an internship, I got appointed as an intern in the Qatar Islamic Bank. The organization titled me as CSR (customer service representative), where I had multiple tasks to manage like dealing with clients and communicating with employees for inquiries. It was basically a trainee position to go with which involved skill development, teamwork and active participation. I was part in different activities like client dealing, transactional activity or service work. Officially I was a trainee, but actually I was provided a comprehensive forum where I could explore each important area of the banking nature. It was basically non-conventional banking, giving me a chance to learn that how non-conventional banking works and functions. Overall, it was not a distinctive position like a titled one, but a diverse position, which involved different tasks and different scenarios. Becoming part of the Qatar Islamic Bank system was an ideal learning opportunity.

Sydney Gay And Lesbian Mardi Gras Festival Tourism Essay

Sydney Gay And Lesbian Mardi Gras Festival Tourism Essay Introduction Sydney is the capital of the New South Wale of Australia, and it is best known as a vivid, charming and lively city and with its famous icons, such as Harbour Bridge and the Opera House. In addition, Sydney has started to build a new image of gay capital of the South Pacific (Kates, 2003). Since the late 1970s, Sydney has begun to hold Gay and Lesbian Mardi Gras every year during end of February and early March and has also become a holiday destination for lesbian and gay tourists. There are various cultural activities and entertainments that allow participants to have fun and allow visitors to have a better understanding of gay and lesbian culture while they are celebrating the liberty of homosexual behaviour. Activities included film festival, community events, a fair day of local merchants, sporting events, art exhibits, dance parties, theatre productions and parade (Kates, 2003). The parade is hold in the CBD of Sydney; the festival normally goes for three and a half week. In this paper, the issues on social and economic impacts that have occurred by the Sydney Gay and Lesbian Mardi Gras festival will be discussed in detail. Social Impact Social impact of Sydney Mardi Gras can be divided into positive impact and negative impact. For the positive impacts are community cohesion identity, entertainment socialisation opportunities and community growth and development. In opposition, negative impacts are inconvenience and crime or bad behaviour. According to the research information, which is done by the University of Melbourne (Kelley, H, 2001), the data has shown that back in 1984, homosexual behaviour is not yet be accepted morally. However, Australians attitude toward homosexual started changed dramatically after 1984. The survey it has been done in the middle 1980s, 64 per cent of Australian believe that homosexual behaviour is always wrong, however, in the 21th century (the survey was done in 2001, data might change after years), the per cent drop from 64 to 48, and the percentage for the answer Not wrong at all rise from 16 per cent to 28 per cent. Even though there are still a group of people who against toward homosexual behaviour, however, one thing can be pleased is that Australians attitude toward homosexual become friendlier each year, this can be proof by the number of participants in The Sydney Gay and Lesbian Mardi Gras festival. The number of participants and visitors increase rapidly from hundreds of people to hundred thousand and more and more people start to pay attention on the event. When the people in Sydney start to celebrate homosexual behaviour in 1978, and the size of the event become bigger each year, this witness the transformation of people in Sydneys attitude on homosexual behaviour, which representing the people of Sydney possess a positive attitude toward homosexual behaviour, and will assist the city of Sydney to build a positive image for gay and lesbian tourists, since the tourist believe that Sydney does not have any discrimination on homosexual behaviour, instead of that Sydney community is welcome and willing to celebrate it (Markwell, 2001). Additionally, the festive give individuals a chance to embrace their identity as gay or lesbian, likewise, the local community also embrace their open thought on homosexual behaviour. Every year, not only the people in Sydney, but also with people coming from all Australia, they gather together to celebrate what they believe in and offering gays and lesbians an opportunity to speak out to other societies (Tourism Australia, 2009). One of the marchers who is interviewed by ABC News (2009) said But tonight were just coming out and having a bit of fun in showing everyone the gay and lesbian community is part of the Australian community. The Sydney Mardi Gras Festival is a popular and successful event that has an ability to attract people from other city or even other country to come to Sydney to join the event, it all has to give the credit to the local community, the people of local community provide a great support of the event. Every year, there are thousands of volunteers and local community group to assist organising the event. In order to create a fascinating event, volunteers and community group members has to be fully prepared; therefore, they have to develop special skills in order to be qualified to do jobs. According to the office website of the Sydney Mardi Gras (2010), it says that specific skills are needed for the event such as RSA (Responsible Service of Alcohol), a heavy vehicle or forklift drivers license and experience in communications. Furthermore, the Sydney Mardi Gras is not only celebrating and entertaining, but also it also takes charity seriously. Sleaze Ball is one of Sydney Mardi Gras eve nts; it is specially design for fundraising, the money donated from people who join the event will goes to the charity. Since Sydney Mardi Gras has been a successful event, it attracts many tourists from other states and countries (tourism will be discussed in more detail on economic impact section). Even though, people who come to join Mardi Gras are all from different culture backgrounds and religion, however, they are all passion on the same thing and believe. It is a great opportunity for them to meet new people from different culture background, and they can share they thought, experience and believe, and also allows them to look homosexual behaviour from different cultures perspective view. For all of events, there are always some positive and negative impacts; one of the negative social impacts is inconvenience. During the event, especially larger scales event, several issues need to be considered such as traffic, road closures, noise and crowding. Since the number of people participates in Sydney Mardi Gras festival is huge, which means that if the crow management and parade route is not well design, this will be a serious issue on the day of the event. Since majority of the event venue and the parades routine are in the CBD, and the road closures will influence the traffic in the event surrounding area. As a result, traffic jam will occur and since several roads are close for the event, this will make the people detour to avoid the event. In addition, the main Parade Route is in Oxford Street and Flinder Street, and there are residential apartment within this area. (Sydney Traffic Committee, 2009)The field notes that are taken by Steven M. Kates (2003), he states tha t 20 gay men and lesbian celebrants in the hotel room as they watched the parade from the two balconies, drank alcohol, talked, laughed, and consumed illegal drugs during the evening. During the parade, the noise population will be local residents temporary issues. Another negative social impact is crime or bad behaviour. There are many entertainments and activities in the Mardi Gras Festival; one of the highlight of the festival is Mardi Gras party. According to a field note that are taken by Steven M. Kates (2003), in his journal, he mentions that Drugs such as speed, cocaine, and Ecstasy provide the energy for dancing to rhythmic techno music until the early hours of the moming at the Mardi Gras party and connecting with the bare-chested, sweating men there. According to John p. Walters (2000) paper, it states that drugs and alcohol is often related to crime, but drugs and alcohol always used by people who is seeking for more excitement. However, drugs abuse and alcohol will lead to behaviour disorder; people losing their ability to control themselves, this will result in rise in the crime rate, such as generating violence, steal, sexual assault and robbery. HIV is another serious issues derive from drugs and alcohol, unprotect sexual act wi ll increase the risk for transmitting HIV, and the people who participate in the party, their health will be treated and this will become secret worry for the society. Economic Impact Every year on February, large amount of tourism come to Sydney for the Mardi Gras, ever since Sydney has created an image of international gay and lesbian capital, Sydney has become one of the most popular places for homosexual tourists. Even if the worlds economic may be is facing recession, but the event scale for Sydney Mardi Gras is getting larger each year, start from thousand of visitor to most recent one, hundreds of thousands of people come to join the 2010 Sydney Mardi Gras Parade. ABC News has made a comment on 2009 Sydney Mardi Gras, it saidà ¢Ã¢â€š ¬Ã‚ ¦the Sydney Gay and Lesbian Mardi Gras provided the perfect antidote to economic doom and gloom. The Sydney Mardi Gras not only is not affected by the economic, conversely, the event is beneficial for the local economy. The major economic impact for Sydney Mardi Gras is increase income due to the growth of tourism market, other impact such as operating cost for event preparation. Firstly, each year, the Australia government estimated the Sydney Mardi Gras will contribute on nation economy net income approximate tens of millions of dollars (Marsh and Levy 1998). Even though the Sydney Mardi Gras itself may not make any profit, however, it certainly affecting the local economy by attracting domestic and international tourists to come to Sydney. Markwell (2001) stated that during the end of February, domestic and international tourists are attract to Sydney for Mari Gras; the peak period for this season is days around the parade and parties at the end of festival. The majority target market for Sydney Mardi Gras are tourists from United States, Canada, the Netherlands, New Zealand, France, Germany and the United Kingdom. Moreover, this market is also growing in the Southeast Asia, the number of the tourist from Southeast Asia region is increasing recently. Tourism Australia (2010) provide the information on the number of tourists for the first Sydney Mardi Gras was approximately 1,500 people, in the most recent Mardi Gras 2010, from the New Mardi Gras Annual Report(2010) states that, total visitors was 21,779 and 8,700 were from overseas. There is no doubt that tourist is good for local economic, according to the annual report for Travel to Sydney (2009), it states that in 2009, visitor spends average $236 per night when they are in Sydney, it is only include accommodation, transportation and activities such as eat out at restaurants, sightseeing or shopping. Markwell (2001) also mentions some examples of expenditure that tourists will be spend on the Sydney Mardi Gras festival. He states that there are 80 events at the festival and about 15 percent of it is free, some popular events require tickets to entry. The event such as dance party, it cost AU$114.00 in 2001. It is quite expensive if a person wants to attend all of events of Sydney Mardi Gras, especially, some events require special outfits, therefore if a couple want to attend the event, the expense on buying outfit, waxing, tanning, gym training, hairstyling, and party drugs will cost a lot of money. Attending all events already costed fortune, for tourists, there are extra costs for them, such as accommodation and transportation. Markwell (2001) also states that homosexual tourists are willing to spend all those money; because of Mardi Gras events can assist them to define their gay and lesbian identity. In 2010 Sydney Mardi Gras, there were 8,700 people from overseas, the Sydney local government would at least earn 2 million per a day just from the Mardi Gras visitor. C. Michael Hall (1996) believes that in order to promote an event or a place to tourists as their destination for vocation, it is very important to leave a good impression and unforgettable experience. To achieve that, event has to be fully prepared and organised. Since the scale of Sydney Mardi Gras is huge, to produce a memorable, fantastic event require a huge amount of fund, therefore, government support and sponsor are essential. According to New Mardi Gras Annual Report (2010), it states that for 2010 Sydney Mardi Gras sponsorship income was over $800,000 and government supporting fund was $300,000. That money will flow to the local community eventually that can stimulate the local economic growth. Additionally, an huge scale event such like Sydney Mardi Gras need a certain amount of workforce, even though there are thousands of volunteers each year, however, there are always job vacancy, which can improve the local unemployment rate. As the paper has mentioned earlier, the local government cannot expect to make profit out of the Sydney Mardi Gras Festival. The revenue for 2010 Sydney Mardi Gras is 4,241,283 and expense is 4,826,910, apparently, the outcome for this year is deficit, the event organisation does not make any profit out of this event. However, looking in long-term, the Sydney Mardi Gras has assist Sydney to build an image global gay-friendly destinations (Markwell, 2001), this result in expansion on homosexual tourism market. Conclusion In conclusion, this paper has discussed how the Sydney Gay and Lesbian Mardi Gras has become an important role in the Sydney major event. As it have a strong impact on Sydneys social and economics dimensions and it is recently referred as tourism icon by Tourism New South Wales. Nowadays, Sydney is known as homosexual friendly city. The Sydney Mardi Gras has impact on social and economic on both positive and negative ways. It is excellent that people in Sydney is open-mind with homosexual behaviour, they are not only possess positive attitude toward it, but also willing to embrace them and celebrate it with wonderful events. However, social issues such as inconvenience and crime or bad behaviour are happen every year which may harm the local communitys life quality. Moreover, economic impacts mostly are positive; the Sydney Mardi Gras assist Sydney to expand its tourism market, and in the event period, thousands of people come to Sydney for Mardi Gras, it is beneficial for local econ omy. Even though, it is difficult to make profit out of the event, however, in long term, Mardi Gras has already assist Sydney to built its reputation in tourism market. Overall, Sydney Mardi Gras

A Quantitative and Qualitative Look at Southwest Airlines and British Airways :: Airlines Marketplace Aviation Benchmarking

A Quantitative and Qualitative Look at Southwest Airlines and British Airways In today's competitive marketplace, all firms are seeking ways to improve their overall performance. One such method of improvement, recently adopted by many firms, is benchmarking. Benchmarking is a technique used to evaluate internal business processes. "In this analysis, managers determine the firm's critical processes and outputs, baseline those processes, then compare the performance of each process against a standard outside the industry" (Bounds, Yorks, Adams, & Ranney 1994). To effectively improve a business process to world-class quality, managers must find a firm that is recognized as a global leader, not just the industry standard. Successful benchmarking requires tailor-made solutions, not just blind copying of another organization. Measurement and interpretation of data collected is the key to creating business process solutions. "Benchmarking's real role has to be seen in the context of the organization that is continuously implementing improvement" (Bendell, Boulter, & Goodstadt 1998). Organizations implementing the benchmarking process are continuously looking to improve, and planning improvement. Improvements can be made by looking at the firm both internally and externally. Internal improvements are implemented by analyzing processes and setting targets for performance. However, output performance measures are not able to help management understand why a practice is effective. This understanding is a result of personal interpretation of the process. Organizations must look to other firms for ideas to borrow from global leaders, regardless of the scope of the necessary improvement. Equally important as data collection is the actual implementation of the newly acquired business practice. The most important aspect of benchmarking is to enable companies to employ the best business practices. This fundamental theory cannot be overstated. Global competition is growing due to the technological boom. The expansion of the Internet and digital communication has forced once domestic firms to consider foreign competitors. To remain ahead, companies are realizing they must match or exceed the business practices of the best in the world. "The only way that we can drive our organizations to excellence is to ensure that we keep our eyes on our competitors and world best practice in all aspects of business" (Bendell, Boulter, & Goodstadt 1998). Benchmarking should not be considered simply a tool of management, but rather an integral part of the business strategy of a firm. When implementing benchmarking, management must consider the overall issues of performance and process re-engineering. A Quantitative and Qualitative Look at Southwest Airlines and British Airways :: Airlines Marketplace Aviation Benchmarking A Quantitative and Qualitative Look at Southwest Airlines and British Airways In today's competitive marketplace, all firms are seeking ways to improve their overall performance. One such method of improvement, recently adopted by many firms, is benchmarking. Benchmarking is a technique used to evaluate internal business processes. "In this analysis, managers determine the firm's critical processes and outputs, baseline those processes, then compare the performance of each process against a standard outside the industry" (Bounds, Yorks, Adams, & Ranney 1994). To effectively improve a business process to world-class quality, managers must find a firm that is recognized as a global leader, not just the industry standard. Successful benchmarking requires tailor-made solutions, not just blind copying of another organization. Measurement and interpretation of data collected is the key to creating business process solutions. "Benchmarking's real role has to be seen in the context of the organization that is continuously implementing improvement" (Bendell, Boulter, & Goodstadt 1998). Organizations implementing the benchmarking process are continuously looking to improve, and planning improvement. Improvements can be made by looking at the firm both internally and externally. Internal improvements are implemented by analyzing processes and setting targets for performance. However, output performance measures are not able to help management understand why a practice is effective. This understanding is a result of personal interpretation of the process. Organizations must look to other firms for ideas to borrow from global leaders, regardless of the scope of the necessary improvement. Equally important as data collection is the actual implementation of the newly acquired business practice. The most important aspect of benchmarking is to enable companies to employ the best business practices. This fundamental theory cannot be overstated. Global competition is growing due to the technological boom. The expansion of the Internet and digital communication has forced once domestic firms to consider foreign competitors. To remain ahead, companies are realizing they must match or exceed the business practices of the best in the world. "The only way that we can drive our organizations to excellence is to ensure that we keep our eyes on our competitors and world best practice in all aspects of business" (Bendell, Boulter, & Goodstadt 1998). Benchmarking should not be considered simply a tool of management, but rather an integral part of the business strategy of a firm. When implementing benchmarking, management must consider the overall issues of performance and process re-engineering.